HIV-1 Genetic Diversity and Transmitted Drug Resistance Frequency among Iranian Treatment-naive Sexually Infected Individuals

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Speech
Abstract: Background and Aim : In recent years, the patterns of human immunodeficiency virus-1 (HIV-1) transmission in Iran have been changing gradually from drug injection to unprotected sexual contact. This study sought to investigate the phylogenetic trends and characteristics of transmitted drug resistance (TDR) mutations of HIV-1 in a population that is mainly infected through homo/hetero sexual contacts.
Methods : Sixty newly-diagnosed antiretroviral-naive individuals with HIV infection living in Tehran were recruited to this survey and among them, 42 subjects were established to be infected through sexual intercourse. Following amplification and sequencing of the main part of the HIV-1 pol region, the phylogenetic and drugresistance mutation (DRM) analysis was successfully performed on these 42 patients.
Results : Phylogenetic analysis showed that the majority of the subjects were infected with subtype CRF35_AD (88%) followed by subtype B with 7.1%, and subtype CRF01_AE with 4.7%. A total of 7.1% of the subjects were found to be infected with HIV-1 variants with surveillance drug-resistant mutations (SDRMs), according to the last world health organisation (WHO) algorithm. All of the identified SDRMs belonged to the non-nucleoside reverse transcriptase inhibitors (NNRTIs) class, including K103N and V106A, which were found in three patients. Two minor HIV protease inhibitor-related mutations (L10I and G73S) were detected in two patients, albeit these mutations are not included in the WHO SDRMs list.
Conclusion : The dominance of HIV-1 subtype CRF35_AD was observed among subjects of this study who were infected through sexual contact. The moderate prevalence of SDRMs (7.1%) in this population emphasises the fact that the risk of treatment failure in HIV infected individuals might be raised in the future and the preventive measures should be considered by health authorities.