Investigation of humoral answers against 2 acute strain of Bacillus anthracis and it,s vaccinal in laboratory animals models

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Anthrax is one of the important zoonotic diseases in the world. This has been caused by Bacillus anthracis. Nowday, different vaccines are available to protect against it which mainly based on 34F2 strain (spore-forming). In order to evaluation of the humoral immunity in infected or vaccinated animals, a fast and reliable serological test such as ELISA must be developed.The aim of this study has been basedon the production of specific poly clonal antibody against Bacillus anthracis two different vaccinal and acute strains of Bacillus anthracis (34F2 and 17jb respectively).
Methods : For this reason, the strains has been cultured on blood agar medium and injected to different rabbit groups in different scales.
Results : The levels of specific polyclonal antibody have been measured by indirect ELISA method. The surface antigens have been prepared by Ultrasonication and used for development of ELISA.
Conclusion : The results shown that antibody produced in higher levels after 30 day offirst immunization when 5 x 105 bacteria were injected to rabbits. The 17jb strains has been noted as best antigen for ELISA method Based on this study, polyclonal antibody was produced and ELISA method has been developed properly. The antibody could be used for diagnostic stuies.