Prevalence fluoroquinolones resistance among campylobacters isolated from diarrhea patients in Markazi province - 2015

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Campylobacter species, especially C. jejuni and C. coli, are the most common bacterial causes of gastroenteritis in humans (1). In these cases, Erythromycin and fluoroquinolones are drugs of choice for treatment (2). Increased fluoroquinolones resistance in campylobacter are a major concern (3). In Iran, limited studies have been determined antibiotic resistance of these bacteria, so investigating macrolide and fluoroquinolones resistance in them is necessary. This study aimed to determine the frequency of fluoroquinolones resistance and to investigate genotypic determinant of fluoroquinolones resistance in campylobacters isolated from the stools of the dysenteric patients admitted to educational and medical centers in Arak, Iran.
Methods : In this descriptive cross-sectional study, diarrheal stool specimens of the patients admitted to educational and medical centers in Arak in the period from May 2015 to Sep. 2015 were collected and after phenotypic and genotypic investigation of campylobacter, resistance to fluoroquinolones were assessed using disk diffusion method. In addition, gyrA genes contributing to fluoroquinolones resistance in campylobacter were also investigated according to method described elsewhere.
Results : Out of 108 dysenteric stool specimens, 28 (25.92%) campylobacter were isolated of which 26 (24.07%) were C. jejuni and 2 (1.85%) were C. coli. Phenotypic resistance prevalence in C. jejuni was obtained as 18 (64.28%) and in C. coli as 2 (7.1%).Frequency of gyrA fluoroquinolones resistance gene was found as 15(83.33%) and 2 (100%) in C. jejuni and C. coli, respectively.
Conclusion : This study showed high fluoroquinolones resistance in campylobacter isolates. This is very useful data for empirical therapy and demanding comprehensive study with more samples size to be accomplished. In addition, this frequency of fluoroquinolones resistant must be taken seriously and needs more attention.