Idenification of Extended-Spectrum Beta-Lactamase-Producing gram- negative bacteria in Nosocomial and Community-Acquired Infections from Urmia, Iran by combind disk method

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : In most of gram negative bacteria resistance against beta-lactam antibiotics is acquired by induction of beta- lactamases production. The extend of drug resistance among the pathogenic bacterias if ignored may render them uncontrollable. In order to evaluate the prevalence of ESBL producing gram negative bacteria and study antibiotic resistance pattern, this study was carried out at private hospitals in Urmia, Iran.
Methods : During April- August 2015 a total of 330 different biological specimens were collected from patients at different wards of private hospitals in Urmia, lran. Patient,s demographic and clinical data were recorded on prepared questionnaire. All specimens cultured on conventional microbiological media and identified by standard tests. Antibiotic susceptibility testing was performed by disk diffusion method as recommend by Clinical and Laboratory Standards Institute protocols. ESBLs detection was carried out by combined disk method using ceftazidime, ceftazidime + clavunate and amoxicillin, amoxicillin + clavunate and ticarcilin, ticarcilin+clavunate disks.
Results : According of results, majority of specimens were urine (75.0%) which is followed by sputum (15.7%), blood culture (4.8%) and so on. E. coli, P. aeruginosa and Klebsiella were the most prevalent bacteria among the isolates. Antibiotic susceptibility testing revealed that imipenem (65.2% sensitivity) was the most effective antibiotics against the bacterial isolates which is followed by amikacin (62.9% sensitivity) and gentamicin (55.3% sensitivity) respectively. ESBLs screening by combined disk method showed that overall 149 (45.15%) of the isolates were positive.
Conclusion : Our findings showed that the majority of gram negative bacteria in this study were ESBLs producing isolates. Since this kind of resistant can be spread in hospital environment and remain as source of nosocomial infections, it‘s important to screen all multiple drug resistant bacteria for production of ESBLs in order to reduce nosocomial infections and appropriate treatment.