Prevalence of virulence genes in Escherichia coli isolated from children with urinary tract infection in Sanandaj, Iran

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Urinary tract infection (UTI) is one of the most common bacterial infections in children. The most common cause of UTI in children is uropathogenic Escherichia coli (UPEC). The UPEC strains harbor many virulence genes that promote UPEC pathogenicity. The present study was per¬formed to determine the antibiotic resistance and virulence determinants of UPEC isolated from children.
Methods : This cross-sectional study was performed on 32 E. coli strains recovered from urine samples of children with UTI aged 0 to 12 years between April and June 2015 in Sanandaj, Iran. The isolates were examined by PCR for the presence of virulence genes encoding haemolysin (hly), cytotoxic necrotizing factor type 1 (cnf1), P-fimbriae (pap), and afimbrial adhesin (afa). Sensitivity to antibiotics was determined using the disk diffusion method.
Results : Nitrofurantoin and trimethoprim/sulfamethoxazole were the most and least effective antibiotics with susceptibility rates of 96.9% and 21.9%, respectively. The prevalence of genes encoding for adhesins was 25% for pap, and 15.6% for afa. The hly and cnf genes encoding for toxins were amplified in 15.6% and 25% of isolates, respectively. The strains isolated from hospitalized patients displayed a greater number of virulence genes in comparison to isolates from outpatients. Different patterns of virulence genes were identified.
Conclusion : These data show the need for monitoring of drug resistance and its consideration in the treatment of E. coli infections. Investigation of bacterial pathogenicity associated with UTI may help to a better medical intervention and management of UTI.