Evaluation of the effect of antimicrobial activity of the aqueous and alcoholic extracts of Marzeh Kohi and Qazyaqi against Extended-Spectrum-β-Lactamases (ESBL) producing Escherichia coli

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Infectious diseases are responsible for thousands of worldwide early death. Many commercial antibiotics have been used to control human's infectious diseases all over the world. Marzeh Kohi and Qazyaqi wild growing plants of Labiataeand and Water plantain family respectively, are native to the region of Kurdistan Province (Iran). These plants, especially Qazyaqi plant, are utilized by the traditional medicine for the treatment of bladder ulcers, bloody diarrhea, kidney stones, and other types of urinary problems. Urinary tract infections are usually treated via using antibiotics. However, there are an increasing number of reports on the resistance of pathogenic bacteria to antibiotics. More than 150 types of ESBL are reported from different countries which are generally produced by Enterobacteriaceae bacteria.
Methods : This study aimed to investigate the antimicrobial effects of the aqueous and alcoholic extracts of Marzeh Kohi and Qazyaqi on both the standard strain of Escherichia coli bacteria ATCC 8739 (PTCC1330) and clinical isolates of Escherichia coli and the Compared with therapy Antibiotics. The confirmation of the ESBL producing strains was done by the double-disc synergy tests and the broth dilution method was used for the determination of the antimicrobial parameters (MIC and MBC) on standard strain of Escherichia coli and ESBLproducing strains. 14 different extracts of both plants in the different extraction solvents (aqueous solvent,Methanol and ethanol) and different ratios were examined.
Results : The results of this study showed that of all the extracts, the aqueous extract of Qazyaqi and Marzeh Kohi had the highest level of production efficiency, especially Qazyaqi With the largest diameter halo,on standard strain :15/6 and on ESBL strain :12.6, Has had the greatest impact on both bacterial strains. Plant extracts showed inhibitory activity against ESBL-E.coli with varying magnitudes and these effects were dose dependent manner. The highest levels of MIC and MBC Various herbal extracts were observed in ranges from 0.015625 (1/64) to 0.0625 (1/16) mg/ml, Relates to Qazyaqi aqueous extract and 0.015625 (1/64) to 0.125 (1/8) mg/ml Relates to Marzeh kohi Soxhlet methanol extract and the least MIC value was observed by the extract of Marzeh kohi Soxhlet Ethanol extract against ESBL- Ecoli (0.025 (1/4) mg/ml).
Conclusion : There is need for a correct and reliable phenotypic test to identify ESBL beta lactamases and also these bioactive plants may help alleviate the problem of drug resistance. The presence of these chemical constituents in this plant is an indication that the plant, if properly screened using additional solvents, could yield drugs with pharmaceutical significance.