The role of classI and II integrons in multidrug resistance of extended-spectrum β-lactamase-producing Escherichia coli

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Integron is an important factor in the making of multi-drug resistance (MDR) in bacteria. This study evaluated the role of class I and II Integrons in the making of resistance in the extended-spectrum beta-lactamase producing (ESBL) Echerichia coli.
Methods : A total of 66 strains of ESBL-producing E.coli were isolated from urinary tract infection in Kermanshah and their antimicrobial susceptibility was determined. The frequency of class I with its associated gene casts and class II Integron were determined in isolates by PCR. Data were analyzed using statistical methods
Results : Of 66 ESBL producing isolates, 65 isolates (98.5%) were multidrug resistant. The most antibiotic resistance of isolates was to ampicillin (98.4%), ceftriaxone (98.4%), cefotaxime (95.4%) and Co-trimoxazole (86.3%). But all isolates were sensitive to imipenem. The frequency Class I and II Integrons in isolates was 87.6% and 53.6%, respectively. The relationship between class I integron and resistance to tobramycin (p=0.021) and cefotaxime (p =0.01) was statistically significant. Five patterns of gene cassettes were determined in class my intern.
Conclusion : Imipenem is the most effective antibiotic for ESBL-producing E. coli. The frequency of class I integron and with a lower rate for class II integrin have correlation with the antibiotic resistance in E.coli isolates. So that there is a statistically significant correlation between resistance to tobramycin and cefotaxime and the presence of class I integron in E.coli.