The phylogenetic classification of E. coli bacteria isolated from urinary tract infections in central regions of Guilan Province

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Urinary tract infections are the most common infectious diseases. Today the E. coli bacteria has been reported as the most dominant factor causing urinary infection in 80 to 90 percent of the patients. Due to the increasing use of antibiotics and subsequently drug resistance, this study was conducted to evaluate the pattern of antibiotic resistance of E. coli strains isolated from patients with suspected urinary infection and phylogenetic classification of these strains.
Methods : In this cross-sectional descriptive study, the number of 100 isolates of E.coli from patients with urinary infection was collected from five laboratories in Rasht city. The isolates were confirmed using common morphological and biochemical tests. The genomic DNA of isolates was extracted and the pattern of phylogenetic groups was performed using Multiplex-PCR method. The results were analyzed using Fisher's exact test. Also the evaluation of antibiotic resistance pattern of isolated strains was done.
Results : In this study the distribution of phylogenetic groups of B2, D, A and B1 among isolates was 64 percent, 24 percent, 12 percent and zero percent respectively. Also the highest rate of antibiotic resistance was to Cefotaxime (84 percent), Piperacillin and Cefixime (80 percent). The lowest rate of antibiotic resistance was to Imipenem (8 percent), chloramphenicol and gentamicin (12 percent).
Conclusion : E.coli isolated from urinary tract infections in this region, most belong to the phylogenetic group of B2.