Coexistence of Class 3 and Class 4 Integrons in Escherichia coli Samples Isolated from Patients

نهمين كنگره بين المللي آزمايشگاه و بالين و دومين كنگره ملي علوم پايه پزشكي و توليد دانش بنيان

3 الي 6 اسفند 1395، تهران - ايران

Presentation Type: Poster
Abstract: Background and Aim : Multidrug resistance (MDR) is an increasing problem in all countries. Resistance genes that is presented in MDR bacteria, encoded on integrons, which are mobile genetic elements and transferred MDR. The aim of our study is detection of class 3 and class 4 integrons in Escherichia coli samples isolated from patients (from November 2013 to April 2014, Kurdistan Provience, Sanandaj, Iran).
Methods : Polymerase Chain Reaction (PCR) and specific primers were used to detect int3 and int 4 genes within 120 isolates of E. coli that were obtained from clinical specimens of patients. Data were analyzed using SPSS16 and chi-squared test (p<0.05).
Results : The results showed a coexistence of class 3 and class 4 integrons between 3/120 (2.5%) of E. coli isolates. There was no association between class 3 and class 4 integrons and MDR bacteria (p>0.05).
Conclusion : Our data demonstrate the present of class 3 and 4 integrons that are a reason for transmission of MDR. So identification of class 3 and 4 integrons in E. coli and reservoir of them is necessary for prevention of MDR bacteria.