Sequencing of HIV gag-pol region genome of HAART Long Treatment Experienced with Stable Clinical QualificationPatients
3 الي 6 اسفند 1395، تهران - ايران
Presentation Type: Poster
Abstract: Background and Aim : The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS). Human immunodeficiency virus type 1 is a complex retrovirus encoding 15 distinct proteins. Substantial progress has been made toward understanding the function of each protein, and three-dimensional structures of many components, including portions of the RNA genome, have been determined; the Gag and Env structural proteins MA (matrix), CA (capsid), NC (nucleocapsid), p6, SU (surface), and TM (transmembrane); the Pol enzymes PR (protease), RT (reverse transcriptase), and IN (integrase); the gene regulatory proteins Tat and Rev; and the accessory proteins Nef, Vif, Vpr, and Vpu.
Methods : To understand the molecular epidemiology of HIV-1 infection in Iran, we conducted the study to analyze the genome sequence of gag-pol region of Iranian HIV-1 isolates. For this cross-sectional study, we enrolled 2 HIV-1-infected individuals associated with HAART drug use. Near gag-pol genome sequences obtained from their plasma samples that were analysed by RT nested PCR. Then the sequences were used for phylogenetic tree and drug resistant analyses.
Results : Phylogenetic analyses showed that among 2 isolates, 2 were clearly identified as CRF35_AD,after that proteins of this sequences were compared with RefSeq NC001802.1 and one other 35_AD virus for aminoasids and 3D structure differences.
Conclusion : Because of stable clinical qualification of patients, drug resistance analyzes did not show any important resistant to antiviral drugs except for 2points of one of our patients.