Scrophularia oxysepala medicinal plant extract exert anti-cancer activities on the mouse breast cancer cell lines by targeting apoptosis

اولين كنگره بين المللي تازه هاي پيشگيري، تشخيصي و درماني سرطان پستان

18 الي 20 آبان 1395، تبريز - ايران

Presentation Type: Poster
Abstract: Introduction: Medical plants have been intensively studied as a source of antitumor compounds. The antitumor effects of the Scrophularia oxysepala medicinal plant extract is not studied on the mouse fibroblast L929 and 4T1 mouse breast cancer cell lines. In the present study, cytotoxic effects of the Scrophularia oxysepala extract were investigated on viability of L929 normal cells and 4T1 mouse breast cancer cell lines.
Material & methods: The cytotoxic effects of Scrophularia oxysepala on 4T1 and L929 cells were studied using MTT assay, Trypan blue staining, and DNA fragmentation assay were done at selected concentrations of the plant extract.
Results: According to the findings, the Scrophularia oxysepala medicinal plant extract (stems and leaves) can alter 4T1 cells morphology. So the Scrophularia oxysepala extract inhibits cell growth albeit in a time and dose dependent manner and results in degradation of chromosomal DNA. Cytotoxic effects of Scrophularia oxysepala extract on the 4T1 cells were significantly higher than on the L929 cells.
Conclusion: Our data well established the anti-proliferative effect of Scrophularia oxysepala extract, and clearly showed that the plant extract can induce apoptosis in vitro, but the mechanism of its activities remained unclea
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