suppression of matrix metalloproteinases -2 and -9 in murine breast cancer cell line by β-d-mannuronic acid

اولين كنگره بين المللي تازه هاي پيشگيري، تشخيصي و درماني سرطان پستان

18 الي 20 آبان 1395، تبريز - ايران

Presentation Type: Poster
Abstract: Introduction: Proteases, especially matrix metalloproteinase (MMP)-2 and -9 play key roles in advanced-stage breast cancer invasion and metastasis.4T1 is an animal model cell line for human breast cancer studies, which is able to produce highly metastatic tumors in BALB/c mice. β-dmannuronic acid (M2000) is an anti-inflammatory agent derived from brown algae. In this study, the effect of β-d-mannuronic acid on the cell proliferation and activity of MMP2, 9 in 4T1 cell line was investigated.
Materials and Methods: 4T1 cultured in the presence of various concentrations (0, 5, 25, 50, 100 and 200 μg/ml) of β-d-mannuronic acid. Analysis of tumor cells proliferation and MMPs activity in supernatantswere carried out by LTT assayand gelatinasezymography respectively.
Results: Our data demonstrated that β-d-mannuronic acid did not effectively reduced the cell viability and number of 4T1 in 48h. Howeve r, markedly reduced the activity of MMP2 and 9 in a dose‑dependent manner.
Conclusion: These findings suggest that the therapeutic potential of M2000 in treatment of tumor cell metastasis in breast cancer.