Presentation Type: Poster
Abstract: Background:Fasl is a key ligand in cell surface which acts in Fas/Fasl system and therefore triggers apoptosis, which is a self-destructive mechanism to maintain homeostasis. Functional polymorphisms in this gene were proposed to be associated with breast cancer risk. Breast cancer is one of the most common cancers and leading cause of cancer-related death among women worldwide. In present study, we focused on two polymorphisms of Fasl gene to analyze the association between haplotypes of these SNPs and breast cancer risk. These polymorphisms were Fasl –844 T/C (rs763110) and FASL INV2nt -124 A/G (rs5030772).
Material and method: The association between these two polymorphisms and breast cancer risk, was examined by a case-control study on 70 patients with breast cancer and 70 healthy controls from East Azarbaijan population. At first, genomic DNA isolated from peripheral blood of case and control individuals using salting out method. Then, Genotyping was performed by RFLP-PCR and ARMS-PCR (restriction fragment length polymorphism and amplification refractory mutation system respectively) methods. The data were analyzed by SHEsis platform, using pearson test, with a significance level of 0.05.
Results: Frequency of (CA) haplotype was higher in patients than control individuals (65.35 and 51.57 percent respectively) (OR=1.501, 95%CI=0.931-2.421, P=0.094768). frequency of (CG) haplotype was higher in healthy group than in patients (11.43 and 1.65 percent respectively) (OR=0.134, 95%CI=0.026-0.698, P=0.005593). (TA) haplotype frequency in case and control individuals was 40.65 and 30.43 percent respectively (OR=1.473, 95%CI=0.856-2.534, P=0.160798). (TG) haplotype frequency in case individuals was lower than in controls; 32.32 and 46.57 percent respectively; (OR=0.603, 95%CI=0.356-1.022, P=0.059052).
Conclusion: In our study, there was association between (CG) haplotype and decreased risk of breast cancer in East Azarbaijan of Iran.