Expression changes of ARSD and its neighboring lncRNA genes in tissue samples of patients with breast cancer

اولين كنگره بين المللي تازه هاي پيشگيري، تشخيصي و درماني سرطان پستان

18 الي 20 آبان 1395، تبريز - ايران

Presentation Type: Poster
Abstract: Introduction: The incidence age of breast cancer in Iran compared to developed countries have fallen by about a decade. however, an effective method for early diagnosis and/or treatment of the disease has not been found. Further genomic studies about different types of cancer, have shown that aberrant expression of lncRNAs can have an important role in the progression of cancer, metastasis as well as prevention, diagnosis and treatment of breast cancer. In this study, for the first time, the expression levels of ARSD and its adjacent lncRNA that is called ARSD-AS1 have been investigated in the clinical samples of tumor tissues of breast cancer and their non-tumor margins.
Methods and Materials: In this study, 36 tumor samples and their non-tumor margins were collected from patients with breast cancer. Total cellular RNA were isolated from each sample separately. Then, cDNA synthesized and Real-Time PCR technique were performed for quantitation of ARSD and ARSD-AS1 genes relative to GAPDH as internal control in clinical samples. Data was analyzed and Relative expression of ARSD and ARSD-AS1 genes in clinical samples of breast tumor tissues compared to non-tumor margins of the same tumors was showed.
Results:The results indicated that ARSD gene in tumor specimens compared to non-tumor tissue margins of the same tumors upregulates significantly. Also, ARSD-AS1 gene in tumor specimens compared to non-tumor tissue margins down-regulates significantly. Previously, the relationship between the expression of ARSD and its adjacent lncRNA had not been studied in breast cancer.
Conclusion:The results of this study showed that upregulation of ARSD and down-regulation of ARSD-AS1 genes in tumor tissues comparison to non-tumor tissues of the same tumors are in agreement with EST profiles and the recent results on Next Generation Sequencing, (NGS) studies, respectively. The results of our study may confirm the role of ARSD-AS1 in preventing preserve pluripotency stem cells as well. The results also shown that potential of noncoding RNA ARSDAS1 in therapeu-diagnostic of breast cancer in the future that requires further investigations.
Keywords: