CONSTRUCTION OF RECOMBINANT FUSION FIMCH PROTEIN AS IMMUNOGEN CANDIDATE AGAINST URINARY TRACT INFECTION

The 17 th International and Iranian Congress of Microbiology

23 till 25 August 2016، Tehran - Iran

Presentation Type: Speech
Abstract:

Background and Aim: Urinary tract infection is one of the most common human infections and uropathogenic Escherichia coli strains are the most common factors. These str ains are colonized in the bladder and they cause cystitis, but they can move to kidneys and to be a pyelonephritis factor. Because of the FimH protein in UPEC strains colonization and infection creation have very important role, preparation of a vaccine wi th this adhesin is attractive.
Methods: . In this study, FimH adhesin and their attendant protein that was named FimC was selected as an immunogenic candidate. From Escherichia coli strains 35218, genomic DNA was extracted and fimH and fimC genes were multi ply by PCR method separately. After connecting two pieces together by PCR method, the final product was cloned in the pBluescript vector and was obtained the clone containing fimCH gene by blue - white screening. After that the indicative primers were design ed and after doing PCR method, the resulting product was cloned in pET23a vector. Culture and induction of gene were performed to the culture medium by adding IPTG, by transfer that vector into origami Escherichia coli strains. FimCH recombinant protein ex pression in bacteria was approved by SDS - PAGE and Western blot methods
Results: This recombinant merged protein can be used in Immunogenicity review studies on animal cases.
Conclusion: This recombinant merged protein can be used in Immunogenicity review stu dies on animal cases.