GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS STRAINS DIRECTLY ON CLINICAL SAMPLES BY 24 LOCUS MIRU - VNTR METHOD

The 17 th International and Iranian Congress of Microbiology

23 till 25 August 2016، Tehran - Iran

Presentation Type: Speech
Abstract:

Background and Aim: Tuberculosis (TB) is considered as a public health concern in the world. TB is a chronic infectious disease whose etiolog ic agent is Mycobacterium tuberculosis (Mtb). Molecular procedures are useful tool to control the disease. Prompt genotyping of Mtb can greatly helpful in molecular epidemiology of the disease.
Methods: One hundred and thirty - nine clinical samples from TB patients referring to department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran between the years 2011 to 2016 were selected for this study. Genotyping based on 24 loci MIRU - VNTR proc edure was performed directly on clinical samples.
Results: In the 139 samples studied, one hundred and thirty samples (93.5%) had different pattern. Among loci used in MIRU - VNTR loci of 2401,580 and 577 were amplified more successfully in the most of clinic al samples. Discriminative power of 2401, 2163b and 960 loci had highest variety and differentiation.
Conclusion: Genotype based on MIRU - VNTR procedure is fast and has strong differentiation for genotyping and study of Mtb infection. Performing this procedu re on clinical samples and also use of loci that showing highest variety can create a faster way to get valuable data.