IMM UNOLOGICAL EFFECT OF RECOMBINANT PROTEIN OF HELICOBACTER PYLORI IN FUNCTIONAL OF PERITONEAL MACROPHAGES

The 17 th International and Iranian Congress of Microbiology

23 till 25 August 2016، Tehran - Iran

Presentation Type: Speech
Abstract:

Background and Aim: Helicobacter pylori is a specific pathogen found in the human stomach. The immunomodulatory effect of the fractions of Helicobacter pylori have been investigated and observed. The neutrophil - activating protein (HP - NAP) of Helicobacter pylori is a protective antigen an d a major virulence factor. The aim of this study is to assess the effect of recombinant Hp - NapA helicobacter pylori on nitric oxide (NO) production by mice peritoneal macrophages. Recombinant Hp - NapA helicobacter pylori was produced. Mice peritoneal macro phages were purified   and   cultured.   Different   concentrations   of   recombinant   Hp - NapA   were   used   for macrophage stimulation and evaluation of NO production. Cell survival and production of nitric oxide (NO) (that in all cases also, had received LPS stimulation ) were measured.
Methods: Recombinant Hp - NapA helicobacter pylori was produced. Mice peritoneal macrophages were purified and cultured. Different concentrations of recombinant Hp - NapA were used for macrophage stimulation and evaluation of NO production. Ce ll survival and production of nitric oxide (NO) (that in all cases also, had received LPS stimulation) were measured.
Results: The results elucidated that the stimulate effect of recombinant Hp - NapA on NO release was significant at a dose of 30 μg/ml (P=0.0 1) compared to the control group (P=0.003). MTT production was observed at the same dose in macrophages.
Conclusion: According to our findings, recombinant Hp - NapA do not have a distinct cytotoxic activity, it has a positive effect on NO production by perit oneal macrophages. Therefore, it is proposed that recombinant Hp - NapA can be studied further as Immunotherapy.