Presentation Type: Speech
Background and Aim: Helicobacter pylori is a specific pathogen found in the human stomach. The immunomodulatory effect of the fractions of Helicobacter pylori have been investigated and observed. The neutrophil - activating protein (HP - NAP) of Helicobacter pylori is a protective antigen an d a major virulence factor. The aim of this study is to assess the effect of recombinant Hp - NapA helicobacter pylori on nitric oxide (NO) production by mice peritoneal macrophages. Recombinant Hp - NapA helicobacter pylori was produced. Mice peritoneal macro phages were purified and cultured. Different concentrations of recombinant Hp - NapA were used for macrophage stimulation and evaluation of NO production. Cell survival and production of nitric oxide (NO) (that in all cases also, had received LPS stimulation ) were measured.