CLONING OF MUTANT ESCHERICH IA COLI PHYTASE (R92K) BY SITE DIRECTED MUTAGENESIS
23 till 25 August 2016، Tehran - Iran
Presentation Type: Poster
Background and Aim: Phytases (myo - inositol hexakisphosphate phosphohydrolase) are a class of phosphatase catalyzing the stepwise hydrolysis of phytate to less phosphorylated myo - inositol derivatives and inorganic phosphate, phytate is the primary form of phosphate storage in plants. Phytases are used as an animal feed additive to improve phosphate bioavailability. Among microbial phytase, E. coli phytase has gain commercial application in broiler feed formulation due to high specific activity, acidic pH op timum, and pepsin resistance. This study focused on cloning and expression of mutant E. coli phytase using site - directed mutagenesis.