Competitive RT-PCR coupled with Enzyme-Linked Immunosorbent Assay for Quantification of Human Immunodeficiency Virus Type 1 RNA: Comparison with Commercial Quantitative Assay
22 آبان 1387، تهران - ايران
Presentation Type: Speech
Background: Different techniques have been developed for the quantification of HIV RNA, including competitive reverse transcriptase PCR (RT- PCR), branched-DNA (b-DNA) signal amplification and nucleic acid sequence-based amplification (NASBA). Some of these assays are now commercially available. However, the access to these commercial assays for viral load monitoring in IRAN is still limited due to the high costs. We developed and evaluated a quantitative-competitive RT-PCR-enzyme-linked immunosorbent assay to sensitive measurement of HIV RNA levels in plasma. Results of this assay were compared with the COBAS Amplicor HIV-1 monitor test.