Phylogenetic analysis of HIV-1 pol gene (RT sequences) among Iranian infected patients

دومين همايش ساليانه پژوهش ايدز در ايران

22 آبان 1387، تهران - ايران

Presentation Type: Speech

Background: Human immunodeficiency virus (HIV) as a retrovirus is the cause of the disease known as AIDS (Acquired Immunodeficiency Syndrome). In the current study, we examined the genetic diversity of HIV-1 strains circulating in the city of Tehran, the first largest metropolitan area in Iran.
Material and Methods: Sequencing and phylogenetic analysis of human immunodeficiency virus type 1 (HIV-1) pol gene is a useful method for sub typing strains of HIV-1. All of the reference sequence of HIV-1 has been downloaded from gene bank and alignment was be done. We designed our primers with the Gene runner, Oligo analyzer and the Oligo softwares. HIV-1 RNA derived from plasma of 24 infected patients, from the Imam Khomeini Hospital in Tehran. RT Nested- PCR was developed and the product were extracted from agarose gel. The products were sequenced and phylogenetic analyzed by Mega 4 software.
Results: The developed PCR method could successfully amplified the specific sequence. The analysis of sequences and phylogenetic showed subtypes A1 and B in 14 and 10 patients respectively.
Conclusion: this study is the first phylogenetic analysis of HIV-1 pol gene (RT sequences) in Iran. The results indicated intravenous drug users are infected with HIV-1 subtype A1, hemophiliacs and blood receiver are infected with HIV-1 subtype B. The results also showed that the subtype A1 is the most prevalent in HIV infected patients. Such information can be useful in intervention strategies and planning control.