COMBINED USE OF HEAT-SHOCK PROTEIN 70, GLYPICAN-3 AND GLUTAMINE SYNTHETASE IN DIAGNOSIS OF HBV-RELATED HCC

Hepatitis Monthly

Volume 17 - Number Suppl.1

Article Type: Original Article
Abstract: Background: Hepatitis B infection is one of the most common cause of the hepatocellular carcinoma. Pathological analysis sometimes fails to conclude the reliable diagnosis of hepatocellular carcinoma. The aim of this study is to assess the diagnostic accuracy of a panel of markers (HSP70, GPC3, GS) for the diagnosis of HBV-related HCC in an early stages.
Methods:The expression of HSP70, GPC3, GS were determined by immunohistochemistry and quantitative real-time PCR (q-PCR) methods, using 121 cases from patients with HBV, HCC and HBV+HCC and 30 normal tissues as the control tissue samples.
Results: HSP70, GPC3, GS were expressed in higher levels in HBV-related HCC patients compared to HBV group. The results showed that the labeling index of HSP70, GPC3, GS are correlated with immunohistochemical and molecular expression of HSP70, GPC3, GS. The sensitivity and specificity for HCC diagnosis were 43.4% and 89.7% for HSP70, 64.3 and 90.4% for GPC3, 60.7% and 94.3% for GS, respectively. For HCC detection the overall accuracy of marker combination, regardless of whichone, was 59.4% (3 positive markers) and 74.2% (2 positive markers) with 100% specificity.
Conclusion: The current study demonstrated the clinical usefulness of the combined panel in the diagnosis of HBV-related HCC cases and proper combinations of HSP70, GPC3, GS markers could improve diagnostic accuracy.
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