INVESTIGATION OF THE HBS AG MUTATION PATTERNS CORRELATION WITH DISEASE PROGRESSION IN ASYMPTOMATIC CARRIERS AND HEPATOCELLULAR CARCINOMA/CIRRHOTIC HBV INFECTED PATIENTS

Hepatitis Monthly

Volume 17 - Number Suppl.1

Article Type: Original Article
Abstract: Background: The HBsAg variations not only render immune evasion, but also leave a drastic effect on HBV replication and infection outcome. Regarding controversial issues, the role of HBsAg variations in
Aim: The aim of this study was to compare the pattern of HBsAg mutations among asymptomatic carriers and HCC/cirrhosis patients.
Methods: In this cross-sectional study 19 HCC/cirrhotic patients and 26 asymptomatic subjects were enrolled. After DNA extraction, HBs gene was amplified by using an in-house nested-PCR. PCR products were introduced into bi-directional sequencing. After alignment with reference sequences, the variation patterns of complete HBs gene were investigated.
Results: Regarding sequence alignment some substitutions and stop codon mutations were detected that were significantly different between groups. Substitutions of Y134N/F/L (6) and P120T/S (5) in B-cell epitope were significantly detected in HCC/Cirrhotic group (P<0.05). In opposite, the substitution of Y206S/F/L/H (7) in CTL epitopes was found more frequently in asymptomatic carriers (P<0.05). Totally, 12 Stop codon mutations such as C69*(6) and W182*(2) which resulted in HBs truncation form were found among HCC/Cirrhotic group (P<0.001). Substitutions of Y134N/F/L and P120T/S were more prevalent in HCC/Cirrhotic cases.
Conclusions: Some novel significant mutations were recognized to be associated with disease progression. From them, B-cell epitope variations, stop codon C69* and specific substitutions such as Y134 or P120 in HBsAg might be associated with HCC/cirrhosis progression while mutation at Y206 correlated to asymptomatic state development.
Keyword: