The effects of Hinosan on fertility in the male Balb/C Mice
Introduction: Hinosan is an organophosphate fungicide that inhibits acetylcolinesterase activity, which could be resulted in damages of reproductive organs. This compound has been used extensively in the agriculture, for blast pest control. Therefore, in the present study, we investigated the effect of Hinosan on spermatogenesis in mice. Materials and Methods: For this study, the male mice were divided into three groups. In the experimental group, mice were injected with Hinosan consecutive doses (20mg/kg i.p, five consecutive days per week for one month), sham (corn oil injection) and control (no injection). Animals were scarified 35 days after the latest Hinosan injection. Therefore, the mice testis sections were made and morphologic aspects of testis and spermatogenesis processes were assessed. Data were analyzed using of one-way ANOWA. P-value<0.05 was considered Significant. Results: The Hinosan showed a significant decrease in number of germ cells, spermatocyt, spermatids, Leydig cells, blood vessels and also diameter of seminiferous on testes of the mice decreased. Conclusion: These results suggested that Hinosan is effective on spermatogenesis and seminiferous tubule structure also can exert decrease of germinal cells. Hinosan can induce infertility in mice.