The antioxidantal effect of pomegranate juice on sperm parameters and fertility potential in mice

International Journal of Reproductive BioMedicine

Volume 6 - Number Suppl.1

Article Type: Original Article
Abstract:

Introduction: The antioxidants are defenses against free radicals that can be effective in tackling the problems caused by them and help fertility. Pomegranate juice is among the fruits that has a very high antioxidant effect. This study was an attempt to explore the antioxidant effect of pomegranate juice on sperm parameters (count, morphology, motility) and fertility potential in mice. Materials and Methods: In total 15 male mice were studied with regard to their sperm parameters and fertility potential. Sperms were categorized into three groups regarding to their motility: progressive, non-progressive, immotile. Morphology consisted of normal and abnormal sperms. Mice were divided into control group (n=5) and experimental group (n=10). The experimental group received 20% pomegranate juice for 1 month (duration of spermatogenesis is 1 month in mice). The control group had free access to water. We took one generation from each group to study the fertility rate. After killing the animals, a sample from the tail of epididymal region was taken to test the sperm parameters by light microscope. Results: The results showed that motility and count of sperms didn’t change significantly in both groups. However, the normal morphology and the fertility potential of the experimental group improved significantly. Normal morphology in control group was 68.8±4.76%, and in experimental group was 79.1±6.26% (pvalue= 0.007). The rate of fertility in control group was 5.8±4.08% and in experimental group was 10±1.26% (p-value=0.004). Also the rate of progressive sperms in control group was 35.6±9.91%; while, this rate in experimental group increased to 47.5±11.10% (p-value=0.06). Conclusion: The pomegranate juice is an effective antioxidant that is able to improve the quality of sperm parameters, especially sperm morphology, as well as fertility potential in mice. Probably, intake of this antioxidant by infertile men improves the quality of their sperm parameters.