Assessment of quality control in ART lab using human sperm motility assay (HuSMA)

International Journal of Reproductive BioMedicine

Volume 8 - Number Suppl.1

Article Type: Original Article
Abstract:

Introduction: The quality control (QC) system is used to make sure that disposable items that are used for culture of gametes and embryos are toxic-free. To maintain a high standard in our ART laboratory, all disposable items in ART lab were tested by HuSMA. HuSMA was used as a measure of QC in the ART lab. Materials and Methods: 18 items that are commonly used in IVF lab were tested by HuSMA. Items included gloves, syringes, culture dishes, etc. HuSMA was conducted at 10, 30 minutes and 1, 2, 4 and 24 hours of incubation at room temperature. Sperm motility index (SMI) was calculated by dividing the percentage of progressive motile sperm of the test by the control at the specific time. SMI value < 0.85 was used to indicate sperm toxicity. Each test was repeated 3 times only. Results: QC by HuSMA identified that 3 assessed items, embryo transfer (ET) glove A and B, and puncture glove A were toxic. ET glove A (SMI=0.0) and puncture glove A (SMI=0.0) were toxic after 10 minutes, but ET glove B (SMI=0.63) after 24 hours were shown to be toxic (46% progressive motile sperm compared with control=68%). Also, 2 items, puncture plate A (SMI=0.42) and sperm collection dish (SMI=0.67), were border line after 24 hours, means the results in 4 repeats were different after 24 hours (2 times toxic and 2 times nontoxic). Conclusion: Our study showed that some items used in ART lab may be toxic and their use should be routinely discontinued as part of the QC program. To increase the efficiency of the HuSMA, this assay should be done more than once for each item.

Keyword: ART , HuSMA , QC