Management of physico-chemical factors in an assisted reproductive technology laboratory
Physico-chemical factors of embryos and gametes surrounded milieu including temperature, pH, CO 2 and O 2 pressures and its chemical composition in fallopian tubes and uterus are extremely controlled by different mechanisms. In addition, gametes and embryos usually do not make contact with air and its content in their natural development direction. Our surrounding air contain more deleterious ingredient that could affect normal development and future health of the resulting embryos. Embryology lab and it standard practice has an important role on success of ART cycles and future health of these children. Concurrent Embryos and gametes are extremely responsive and sensitive to chemical and physical factors during their development process, suggesting that a clean environment is apposite in the ART ward and especially embryology lab. Operation rooms in contact with ART labs are high risk areas for production of volatile organic compounds (VOCs) and other air pollutants; there is evidence that VOCs have deleterious effects on quality of embryo and success of infertility treatments. currently it is believed that careful attention should be paid to the air quality and elimination VOCs in embryology lab.The gametes and embryos should be cultured in media that equilibrated for pH (7.35-7.45) and osmolarity (260-280 mosm/Kg) at 37ºC with 100% humidity and 5-6% CO2 pressure. Culture under oil is suggested because of its physical barrier and it avoids of evaporation and quick CO2 diffusion. In addition, especial grad of oil with the low level of toxicity should be used. In view of the fact that an important part of ART success rate will come from the standard practice in embryology laboratories, controlling of humidity, osmolarity, temperature, gas composition and concentration, or a combination of these elements via QC and QA programs has great impact on the outcomes of this technology.