Effects of different doses of adenosine on sperm motility in infertile men
Introduction: One of the causes of male infertility is lack of proper mobility. One way to improve this parameter is to add adenosine to human sperms in the laboratory. Objective: To determine the effect of different doses of adenosine on sperm motility in the ejaculates of fertile and infertile individuals. Materials and Methods: In this experimental laboratory study, 60 samples of semen fluid of infertile and fertile men who referred to Yazd Research and Clinical Center for Infertility were analyzed according to WHO guidelines. Sperm counts, morphology and motility (fast, slow and immotile) were studied. After swim-up technique, the washed samples were divided into 4 groups, each of doses of 0,2, 5 and 10 mg adenosine. The sperm parameters were evaluated post adenosine addition. Data were analyzed statistically using paired T-test and independent T-test. Results: The initial assessment indicates significant differences in sperm parameters, except for slow motility between two groups. In fertile group, dose of 5 mg adenosine increased sperm count, also increased rapid motility, but lowered rates of sperm immotility, at doses of 5 and 10 mg. In infertile group, sperm count improved at dose of 5 mg adenosine, but it showed no effect of slow sperm motility. 5 mg adenosine also improved rapid sperm motility, but dose of 10 showed no effect. Both 5 and 10 mg adenosine significantly lowered immotile sperm rates. Conclusion: addition of 5 mg adenosine to washed sperms improved in vitro sperm motility of infertile men.