Effect of ubiquinone supplementation on seminal oxidative status of infertile men with oligoasthenoteratozoosperia
Introduction: There is growing evidence that damage to spermatozoa by reactive oxygen species plays a key role in male infertility. The aim of this study was to assess the effect of 3 month supplementation with Coenzyme Q10 on seminal plasma total antioxidant capacity and free 8- Isoprostane levels in men with oligoasthenoteratozoospermia. In a randomized placebo-controlled trial, 60 infertile men aged between 20-50 years with idiopathic oligoasthenoteratozoosperia and at least 1 year history of infertility selected. After giving informed consent, they were assigned to placebo or CoQ10 capsules (200mg/d) using random block sampling. Materials and Methods: For each person general and anthropometric dietary questionnaires were fulfilled. Semen samples were obtained with self ejaculation after 3-7 days of abstinence. Seminal plasma total antioxidant capacity was measured using Colorimetric assay. 8-Isoprostane was measured by enzyme immunoassay. Ubiquinone concentration of was assayed by HPLC. Final analysis was done for 47 patients. Differences among the samples were evaluated by student`s t- test and paired t-test. ANCOVA was used to test for the effect of baseline concentrations as covariate. Results: Coenzyme Q10 levels increased in seminal plasma after supplementation, from 44.74±36.47 ng/mL to 68.17±42.41 ng/mL (p<0.0005). Levels of 8-epi-PGF2 were significantly lower after coenzyme Q10 supplementation than after placebo (22.8±12.8 and 42.5±29.5 pg/mL, respectively; p=0.006). Mean changes of isoprostane concentration show significant difference between two groups (p=0.012). There was no significant correlation between isoprostane and ubiquinone concentration in seminal plasma. There was no significant change in TAC in Q10 group, but there was a positive correlation between TAC and seminal ubiquinone concentration. Conclusion: The administration of CoQ10 may play a positive role in the treatment of oligoasthenoteratozoospermia, probably related to its antioxidant role.