Lidocaine as a potential antagonist for Odontobuthos doriae scorpion venom in mice

Research in Pharmaceutical Sciences

Volume 7 - Number 5

Article Type: Original Article
Abstract:
Background and Aims:Odontobuthos doriae (O. doriae), as a local scorpion in middle and southern parts ofIran, is known as a serious health threat, causing wide rangeof toxicities.α-toxins in its venom, activate Na+channels. Therefore, lidocaine as a Na+ channel blocker,seems to be a good candidate to reverse most of itsside effects. Thus, in this project, the efficacy of lidocaine againstthis venom’s lethality is studied.Methods:To calculate the LD50 of compounds, 0-500 mg/kg and 6-12 mg/kg of lidocaine and O. doriae’scrude venom, respectively, were used in 14-days acutetoxicity testing observations. According to finalcalculated LD50s, different safe amounts of lidocaine (25%, 50% and 80% of LD50) were administered at thesame time with different toxic doses of crude venom (80%, 100% and 120% ofLD50). Experiments werecarried out based on 2, 4, 6h and a daily 14-days observations.Results:110 mg/kg and 10 μg/mice are LD50 for lidocaine and crude venom, respectively. Although, 80%LD50 of lidocaine caused 50% decrease in lethality induced by crude venom’s at LD50, decreasing the amountof lidocaine to 50% and 25% LD50, reduced its effects on lethality. However it could reduce many poisoningeffects of the venom. Further, 80% LD50 lidocaine could not reverse the lethality effects of the O.d scorpioncrude venom at 120% LD50 .Conclusions:Although the 80% LD50 of lidocaine decreasesthe O. doriae scorpion venom lethality, butlidocaine’s side effects does not make it as a good candidate for supporting care indication in O. doriae’s venomtoxicity.