Xenotransplantation of Mouse Ovaries into Pseudopregnant Rat Uteri

International Journal of Fertility & Sterility

دوره 9 - شماره Suppl.1

Article Type: Original Article
Abstract:
Background: Xenotransplantation of ovary can help individuals who may be at sterility risk after chemotherapy or radiotherapy of diseases such as cancer. Moreover, this technique can be used to preserve fertility of valuable animal species. The aim of the present study was to preserve balb/c mice ovarian tissue by xenotransplantation into uterus of rat that is immune suppressed. Materials and methods: Seven female Sprague-Dawley rats in proestrous stage were mated with male vasectomised rats and after observation of vaginal plaque they were selected as pseudopregnant seven days after mating. Both ovaries from seven mice in diestrous stage were transplanted into the pseudopregnant rat uteri cavity. Fourteen days after xenotransplantation, the ovaries were removed with the rat uterus, and stained with haematoxylin and eosin for histological examination. Results: Four transplantations were successful and mice ovaries were found in uterine cavities of the recipient rats. However, six ovaries were inactive with no significant secondary or tertiary follicles or corpora lutea, but there was no evidence of tissue necrosis. Two ovaries showed evidence of inactive corpora lutea with primordial follicles. Conclusion: Survival of the transplanted ovaries in the rat uterine cavity demonstrates that mice ovaries can be immunologically acceptable to the pseudopregnant uterine cavity