Biochemical Effectiveness of chestnut seed powder on diabetic rats
Background: Biochemical Effectiveness of chestnut seed powder on diabetic rats Introduction: Diabetes is accompanied by micro and - and macrovascular complications, which contribute significantly to cardiovascular complications in diabetic patients. Nowadays, the interest towards herbal medicine in treatment of diabetes has elevated. We aimed to investigate the effect of chestnut seed powder on liver specific enzymes, cardiac and renal function in streptozotocin (STZ)-induced diabetic rats. Methods: Diabetes was induced in 18 wistar male rats (250-300 gr) by single dose of STZ (60 mg/kg i. p.). Animals were divided into 3 groups, consisting of six animals each, as follows: diabetic controls (group 1), diabetic rats administered 500 (group 2) and 1000 mg/kg per day (group 3) chestnut seed powder orally in aqueous solution. After 4 weeks of administration, a fasting blood samples were taken from all groups to measurement of alanin transferase, aspartat transferas, alkalin transferas, high sencetice-CRP (hsCRP), urea, creatinine and complete blood count (CBC). Results: The study results showed a significant reduction in the hsCRP level in diabetic rats fed with either the 500 mg/kg or 1000 mg/kg chestnut seed powder (p<0.05) compared to diabetic controls . Also, the seed powder- administrated rats showed decreased mean corpuscular hemoglobin compared to diabetic controls [19.33±1.6 pg, 17.70±0.15 pg and 17.51±0.32 pg as groups 1, 2 and 3, respectively; p<0.001. The offspring from diabetic dams administrated with chestnut seed powder showed insignificant different levels of liver enzymes, renal function tests and other CBC tests. Conclusion: The experiments provided evidence to support the cardioprotective effect of chestnut and the health function of chestnut seed against cardiovascular complications dependent on type 2 diabetes.