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A prospective study was performed to evaluate changes in the auditory acuity by pure tone audiometry (PTA) and speech reception threshold (SRT) that might occur in healthy women throughout the menstrual cycle. Forty women with regular ovulatory menstrual cycles underwent PTA and SRT and SRT during the follicular, periovulatory, and luteal phases of the menstrual cycle. No significant auditory perception changes by PTA or SRT was detected during different phases of menstrual cycle. Since hearing acuity seems to be influenced by the variations of ovarian steroids that occur during the menstrual cycle, further studies on more cases is recommended to confirm or rule out these probable changes.