ULTRASTRUCTURAL-LOCALIZATION OF CALCIUM IN NEUROMUSCULAR-JUNCTIONS OF SMOOTH AND SKELETAL-MUSCLES AFTER AMINOGLYCOSIDE ANTIBIOTICS TREATMENT

Histology and Histopathology

Volume 3 - Number

Article Type: ---- Unspecified ----
Abstract:

Aminoglycoside antibiotics are all capable of producing clinically significant neuromuscular paralysis. Since part of the mechanism of action of these antibiotics at neuromuscular junction is a calcium-dependent inhibition of acetylcholine release, so this experiment was carried out in vitro on both somatic (isolated rat phrenic-nerve hemidiaphragm) and autonomic neuro-effector transmission (guinea-pig ileum) using gentamicin and amikacin, to determine the calcium contents at this level. Electron microscopic observations on gentamycin-and/or amikacin-treated materials, using potassium pyroantimonate method suggest a reduction of internal calcium in nerve terminals of both preparations.