Gene polymorphism of saCOL2291 and saCOL2581; candidate genes for vaccine in Methicillin-resistant Staphylococcus aureus

Trauma Monthly

Volume 3 - Number

Article Type: ---- Unspecified ----
Abstract:

Aims: Staphylococcus aureus is an important nosocomial infectious agent. The prevalence of antibiotic resistance complicates thetreatment of staphylococcal infections. Therefore, the development of an effective vaccine against Staphylococcus aureus is important. saCOL2291 and saCOL2581 are potential candidate genes for vaccine development and their sequences are available in Gene Bank. This study was carried out with the aim of determining the mutations in different isolates and confirming the protein of these genes. Materials & Methods: Genomic DNA was extracted from 30 clinical Staphylococcus aureus isolates, randomly obtained from urine, ulcer, mucosa and CSF samples of the inpatients of different Iranian hospitals. The genomic DNA was extracted after culture and the genes were PCR-amplified. The amplicons were sequenced and aligned with Puls4 software. Then, the nucleotide sequences were changed into amino-acid sequences by Gene runner software and the protein sequences were aligned and analyzed. Results: Nucleotide polymorphisms resulted in amino-acid sequence changes. These polymorphisms were located in the N-terminal domains, while the C-terminal domains of these genes were conserved. Conclusion: Both genes are appropriate candidates for vaccine due to their presence in most Staphylococcus aureus species and the existence of conserved C-terminal domain.