The effect of hydro-alcoholic extract of citrus flower on pentylenetetrazole and maximal electroshock-induced seizures in mice

World Applied Sciences Journal

Volume 8 - Number

Article Type: ---- Unspecified ----

The flowers of Citrus aurantium L. are considered to have depressive action upon the central nervous system (CNS). In the present study the effect of hydro- alcoholic extract of Citrus flowers on PTZ and maximal electroshock- induced seizures model was investigated in mice. The animals were divided into 5 groups (n=10), as follows: (1) Saline, (2) Ext 100, (3) Ext 500, (4) Ext 1000 and (5) Diazepam. The animals of groups 2, 3 and 4 were injected by 100, 500 and 1000 mg/kg of extract respectively, 30 minutes before each PTZ injection. The animals of groups 1 and 5 received saline (10 ml/kg) and diazepam (3 mg/kg) respectively, instead of extract. PTZ (90 mg/kg, i.p.) was injected and the animals were observed during 60 min. Behavioral responses of the animals such as latency to first minimal clonic seizure (MCS) and latency to the first generalized tonic-clonic seizures (GTCS) were recorded. In electroshock model, alternating current electroshock stimuli (0.2 sec, 50 Hz, 150 mA) were delivered via saline-soaked, cotton-covered ear-clip electrodes. The duration of tonic convulsion (a tonic extension of the hind limb) was recorded. A significant increase in the MCS latency was seen in Ext 500 and Ext 1000 groups in comparison with Saline group (p < 0.001 and p < 0.01 respectively). The GTCS latencies in Ext 100, Ext 500 and Ext 1000 groups were significantly higher than Saline group. In electroshock model, there was no significant difference between groups. © IDOSI Publications, 2011.