Breast tumor targeting with Te-99m-HYNIC-PR81 complex as a new biologic radiopharmaceutical
Human epithelial mucin, WC I, is commonly overexpressed in adenocarcinoma that includes more than 80% of breast cancers. The PR81 is a murine anti-MUCI monoclonal antibody (MAb) that was prepared against the human breast cancer. We developed an indirect method for labeling of this antibody with Tc-99m in order to use the new preparation in immunoscintigraphy studies of BALB/c mice bearing breast turners. The Tc-99m-PR81 complex was prepared using the HYNIC as a chelator and tricine as a coligand. The labeling efficiency determined by instant thin-layer chromatography (ITLC) was 89.2% +/- 4.7%, and radiocolloides measured by cellulose nitrate electrophoresis were 3.4% +/- 0.9%. The in vitro stability of labeled product was determined at room temperature by ITLC and in human serum by gel filtration chromatography - 88.3% +/- 4.6% and 79.8% +/- 5.7% over 24 h, respectively. The integrity of labeled MAb was checked by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, and no significant fragmentation was seen. The results of cell binding studies showed that both labeled and unlabeled PR81 were able to compete for binding to MCF 7 cells. Biodistribution Studies performed in female BALB/c mice with breast tumor xenografts at 4, 16 and 24 h after the Tc-99m-HYNIC-PR81 injection demonstrated a specific localization of the compound at the site of tumors and minimum accumulation in non target organs. The tumor imaging was performed in BALB/c mice with breast xenograft tumors at 4, 8, 12, 16, 20, 24, 28, 32 and 36 h after the complex injection. The turners were Visualized with high sensitivity after 8 h. The findings showed that the new radiopharmaceutical is a promising candidate for radioimmunoscintigraphy of the human breast cancer. (C) 2008 Elsevier Inc. All rights reserved.