The 14kDa Protein Molecule Isolated from Garlic Suppresses Indoleamine 2, 3-Dioxygenase Metabolites in Mononuclear Cells In vitro

Iranian Journal of Allergy Asthma and Immunology

Volume 4 - Number

Article Type: ---- Unspecified ----

A wide range of biological activities of garlic in vitro and in vivo have been verified including its antioxidant, antitumor and anti-inflammatory; effects. Indoleamine 2,3-dioxygenase (IDO) is an enzyme widely distributed in mammals and is inducible preferentially by IFIN-gamma. IDO degrades the essential amino acid tryptophan to form N-formyl kynurenine. In the present in vitro study, the Modulatory effect of 14kDa molecule isolated from garlic oil IDO induction Was tested. Cultures of mononuclear cells were exposed to 14kDa garlic fraction. Then, their proliferation responses and IDO metabolites were measured. A significant: down-regulatory effect of garlic oil IDO activity was found and also the p proliferation responses of mononuclear cells increased. If these results are verified in vivo, an explanation will be provided on how, garlic may interfere in IDO induction, which paves the way for elucidating its specific therapeutic effect in preventing tumor progress.