Since maleic acid is capable of specially dissolving Chitosan, in this research the method of producing water lubricant nanoparticles in Chitosan solution, based on maleic acid has been studied. The resulting nanoparticles have a remarkable contact surface with the ordinary Chitosan form in absorbing metal ions, proteins and fat existing in Whey. After providing the 0.02, 0.03, 0.05 gr. density of Chitosan nanoparticle and ordinary Chitosan in 10cc Whey with pH 4, 5, 6 were compared in absorbing the particles and the results were observed through (UV) spectrum. Since maximum absorption was observed in pH=6 and 0.05 gr. density of Chitosan nanoparticles and ordinary Chitosan, colloidal absorbent in Whey, by atomic absorption and infrared spectrum were examined in the rest of the test. An increase in the absorption of metal ions like lead, iron and copper is among the significant points of using this kind of nonoparticles. The other items examined in this study are comparison of proteins and fats absorption after treatment of Whey in pH=6 and 0.05gr density of nano Chitosan with ordinary Chitosan in 10cc of this material. © IDOSI Publications, 2012.