Chemo-preventive effects of Diclofenac Sodium: Implication to cancer
INTRODUCTION: Although non-steroidal anti-inflammatory drugs (NSAIDs) appear to be effective in the prevention and treatment of many inflammatory diseases, very little information exists on the comparative effects of common NSAIDs and their unpredictable properties. Regular intake of NSAIDs is associated with a decreased incidence of various types of cancers. This study was designed to evaluate the influence of NSAIDs on a model of cell line.
METHODS: The influence of NSAIDs on fibrosarcoma cell line was investigated using an in vitro cytotoxicity assay, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and gelatin zymography.
RESULTS: Cytotoxicity analysis of diclofenac revealed much higher cell death than other examined agents. A potent apoptotic influence of diclofenac was also confirmed by data obtained from TUNEL assay. Dose-dependent inhibitory effects on matrix metalloproteinases (MMPs) was seen in higher degree in diclofenac compared with the other examined NSAID (piroxicam) and a synthetic glucocorticoid (dexamethasone).
DISCUSSION: Collectively, as induction of apoptosis together with MMPs inhibition could be indicative of cancer treatment, diclofenac might be considered as a chemopreventive agent in cancer.