Erythropoietin Ameliorates Oxidative Stress and Tissue Injury following Renal Ischemia/ Reperfusion in Rat Kidney and Lung

Medical Principles And Practice

Volume 22 - Number 1

Article Type: ---- Unspecified ----

Objective: To study the effect of erythropoietin (EPO) treatment on renal and lung injury following renal ischemia/re- perfusion (I/R).
Materials and Methods: Thirty male Wistar rats were assigned to three groups of 10 rats each. The first group was sham-operated, the second was subjected to re- nal I/R (30 min of ischemia foll owed by 24 h of reperfusion). The third group was subjected to renal I/R and treated with EPO in two doses: the first dose 1 h prior to ischemia (1,000U/kg) and the second dose 6 h after ischemia (1,000 U/kg).
Results: The renal and lung tissue injury index, tissue serum blood urea nitrogen and creatini ne (Cr) were higher in the renal I/R group compared to th e renal I/R + EPO group; the difference was statistically significant (p<0.05). Kidney and lung tissue glutathione peroxidase and superoxide dismutase levels were higher in the renal I/R + EPO group than the renal I/R group; the difference was also statistically significant (p<0.05).
Conclusion: The data showed that EPO pretreatment could be effective in reducing renal and lung injury following renal I/R and could improve the cellular antioxidant defense system. Hence EPO pretreatment may be effective for attenuating renal and lung injury after renal I/R-induced injury during surgical procedures, hypotension, renal transplantation and other conditions inducing renal I/R.